Species: Dog Strain/breeder: Marshall Beagle Sex: Male Age: 15 months Study type: - Treatment: Single dose, i.v. Animal status: Scheduled kill, 24 hours after application Clinical findings: None Organ(s): Stomach Macroscopic finding(s): None Staining: H&E

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Abstract

M. JACOBS¹, F. von LANDENBERG¹ and R.M. GENTA²

¹Institute of Toxicology, Merck KGaA, Frankfurter Str. 250, 64271 Darmstadt, Germany
²Université de Geneve, Geneva, Switzerland

Key words: stomach, parietal cells, degeneration, beagle dogs

In control and treated beagle dogs killed at the end of treatment, a degeneration of parietal (oxyntic) cells of the fundus mucosa of the stomach was detected. The degeneration in H&E stained sections was characterized by eosinophilic, oval bodies either with or without a small dark basophilic "nucleus" surrounded by a halo. The changes were predominantly located in the middle and lower part of the oxyntic mucosa and the degree varied from minimal to moderate. For further characterization of this change special stains (Aurantia, PAS, H⁺K⁺-ATPase antibody) (ITO et al. Tox Path 2000; 28: 304-309), the TUNEL assay and transmission electron microscopy were performed.